Imaging breast cancer evolution in 3D organoid cultures with Luxendo light-sheet microscopy

Bruker's Luxendo light-sheet technology can image a wide range of live, fixed, and cleared biological samples from organoids and embryos to large whole organisms. Overcoming many of the barriers that conventional light microscopy faces, lightsheet fluorescence microscopy, also called selective plane illumination microscopy (SPIM), supports advanced research in the life sciences. SPIM works by de-coupling the fluorescence excitation and detection beams and together with a sheet of light for excitation, selectively illuminates a focal plane for high-resolution images without the danger of phototoxicity. Here, Dr. Martin Jechlinger, the Senior Scientist and Head of VISION Laboratory at the MOLIT Institute, talks about how his lab utilizes light-sheet microscopy to investigate 3D organoid cultures. Specifically, the molecular mechanisms underlying mammary tumor development during breast cancer, as well as what is happening when treatments are failing.