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ImageQuant™ 800 for lateral flow assay development using dyes across the spectrum

Cytiva
4 Jan 2022

The development of assays based on lateral flow has led to low cost and effective large-scale screening of infectious diseases with high specificity. Lateral flow assays consist of membranes, antibodies, and reagents for the detection of a color change or fluorescent dye. Lateral flow assays are an area of intense research and have led to highly robust and sensitive products, including a range of Whatman™ diagnostic solutions from Cytiva. In this application note, Cytiva investigate the use of Conjugated Polymer Nanoparticles (CPNs™) Amersham™ ImageQuant™ 800 systems for lateral flow assays and Western Blotting.

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Amersham ImageQuant 800 systems

Cytiva

Flexible imagers with automated features for sensitive, accurate chemiluminescence and fluorescence detection. To support your electronic data management, an optional GxP module is available, designed to meet increasing requirements of regulations like FDA 21 CFR part 11 and EU GMP Annex 11.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Lateral Flow AssaysConjugationNanoparticlesNanoparticles are between 1-100nm in size. Nanoparticles can be used for a wide variety of applications including biomedical, catalysis and electronics.

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