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Identification, Quanification and Annotation of Heart Membrane Proteins Using Label-Free Nanoscale LC /MS

Waters
3 Jun 2014

Heart diseases resulting in heart failure are among the leading causes of morbidity and mortality in the Western world and can result from either systemic disease (e.g. hypertensive heart disease, ischaemic heart disease) or specific heart muscle disease (e.g. dilated cardiomyopathy (DCM)). Sub-proteome analysis of such disease sub-sets affords a reduction in sample complexity, potentially revealing biomarkers of cardiac failure that would otherwise remain undiscovered. Label-free nanoscale LC/MS is used in this study to validate a Triton X-114 based enrichment method for cardiac membrane proteins.

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IdentityE High Definition Proteomics system

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The Waters IdentityE High Definition Proteomics system defines the new standard for rigorous protein identification. The IdentityE High Definition Proteomics System is an UltraPerformance LC® (UPLC)/High-Bandwidth Mass Spectrometry (MS E) system solution suitable for confident protein identification designed to meet stringent requirements as outlined by leading scientific journals.The new Waters IdentityE High Definition Proteomics System catalogs complex protein digest mixtures containing tens of thousands of peptides. The system features a Waters nanoACQUITY® UPLC System for high chromatographic peak capacity and retention time reproducibility, together with an electrospray tandem mass spectrometer - Waters Q-Tof Premier™ or Waters Synapt HDMS system - for high dynamic mass resolution and consistent over-sampling of complex protein digests – two hallmarks of high definition proteomics. Waters comprehensive peptide ion accounting informatics mine deeper into UPLC/MS E data to identify more peptides/proteins with greater sequence coverage and statistical rigor than conventional LC/MS/MS methods. This system solution is fully supported with quality control standards, defined methods, training and support to ensure reproducible operation and consistent results.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.CardiologyWorld Cup

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