Identification of PI3Kα Inhibitors with an Automated Echelon Fluorescence Polarization Assay using the Labcyte Access Workstation with Integrated Labcyte Echo Liquid Handling Technology
28 Sept 2017Phosphoinositide 3-kinase alpha (PI3Kα) is frequently implicated in human cancer because of its role in the induction of cell proliferation and survival and is highly sought after as a drug development target. High-throughput screening efforts to identify inhibitors of PI3Kα have been widely conducted in the pharmaceutical industry. Compounds identified from high-throughput screening efforts for various targets in the PI3K pathway have developed into potential anti-cancer drugs. The ongoing need to reduce screening costs has led to the growing trend toward miniaturization. The Echo series of liquid handlers combined with the newly released Echo qualified Reservoir affords a reduction in transfer volume of both compounds and bulk assay reagents while increasing throughput and maintaining data quality. This application note describes a fully automated approach utilizing the Labcyte Access Workstation to integrate both the Echo 525 and Echo 555 liquid handlers on the same workstation to facilitate assay execution in the identification of potential PI3K inhibitors
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Echo® 500 Series Liquid Handlers
The Echo® liquid handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source. This product does not use tips, pin tools or nozzles—completely eliminating contact between the instrument and the liquid. The Echo liquid handler provides powerful benefits: Tipless transfer, resulting in dramatic cost savings Improved information about sample integrity and improved transfer quality through measurement of DMSO hydration level Elimination of the need for calibration through Dynamic Fluid Analysis™ technology, a patented Labcyte process Elimination of cross-contamination, carryover or leachates through non-contact transfer Accurate, precise movement of nanoliter-scale quantities, enabling assay miniaturization and further reducing costs Better results through direct dilution of compounds, an improvement over serial dilution methods Greatly simplified assay development and dilution processes through the flexibility to transfer any volume of liquid from any well to any other well