Identification of a rare double positive clone

Given the critical need for rapidly developing genetically edited analytical cell lines to advance drug and disease research, the research community needs solutions for overcoming the bottlenecks preventing simple and efficient development of clonal cell lines. In this case study, using the CellRaft AIR System, Cell Microsystems were able to screen thousands of cells in a single consumable to identify the rare double positive clones, in a matter of weeks, not months. In total, the time from cell seeding to clonal outgrowth was only 16 days, and viable cell banks of the verified clones were cryopreserved in 27 days. Thus, Cell Microsystems shows that the CellRaft AIR® Technology enables successful monoclonal cell line development for even rare or challenging cells faster and more efficiently than traditional methods.