iCell Endothelial Cells: Assaying Cell Proliferation
23 Sept 2012Regulation of endothelial cell proliferation plays a fundamental role in vascular remodeling and angiogenesis in several physiological and pathological conditions. This application note describes the use of iCell® Endothelial Cells, from Cellular Dynamics International, in proliferation assays. iCell Endothelial Cells were treated with a growth factor and/or its receptor inhibitor to modulate a known pro-angiogenic signaling pathway. The proliferation activity was reliably assessed using the CellTiter-Glo® Luminescent Cell Viability Assay, from Promega.
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iCell Endothelial Cells
Cellular Dynamics InternationaliCell® Endothelial Cells, interior surface blood vessel cells derived from human induced pluripotent stem (iPS) cells facilitate vascular-targeted drug discovery, tissue regeneration, and other life science research. iCell® Endothelial Cells exhibit characteristic endothelial gene and protein expression (e.g., CD31, CD105, CD144, ZO-1, and von Willebrand Factor) and endothelial cell functions (e.g., tubular formation, acetylated LDL uptake, barrier function, and wound healing). These cells are an ideal in vitro model system that provides a reliable source of endothelial cells suitable for use in vascular biology research including angiogenesis, atherosclerosis, inflammation, and many other research areas. iCell® Endothelial Cells are shipped as cryopreserved suspensions of dissociated cells with medium supplement, specially formulated for optimal cell performance. iCell Endothelial Cells remain viable in culture for up to 5 passages, thus enabling assessment of both acute and longer-term testing.iCell® Endothelial Cells Benefits: Human Cells - Cells are differentiated from human pluripotent stem cells that provide an easily accessible and physiologically relevant model system for vascular-targeted drug discovery, tissue regeneration and other vascular biology research. Highly Pure Cell Population - Provides endothelial cell-specific responses to reference molecules. Homogenous and Reproducible Fully Functional Model - Cells exhibit standard biochemical characteristics of normal human endothelial cells, and demonstrate utility in a variety of assays including cell migration, cytokine stimulation, tubular formation, and impedance/barrier function. Easy to Implement - Cells are shipped as cryopreserved suspensions of dissociated cells. Simply thaw and use. Acute and Longer-term Testing - Cells remain viable in culture for up to 5 passages, thus enabling assessment of both acute and longer-term testing. iPS Cell-derived iCell® Endothelial Cells Applications: Cells are amenable to a wide array of assays including: Cell adhesion, Cell invasion and migration, Cell permeability, Cell proliferation, Cell viability, Impedance/transendothelial electrical resistance, Tubular formation.