ResourceLife Sciences
iCell Cardiomyocytes: Assaying Caspase Activity and Apoptosis
23 Sept 2012Caspases are critical effector molecules of programmed cell death and are one of the most commonly used markers in the field of mammalian apoptosis. This application note describes how caspsase activity can be detected in iCell® Cardiomyocytes, from Cellular Dynamics International, in response to staurosporine, amoxapine, and emetine using the Caspsase-Glo® 3/7 Assay from Promega. iCell Cardiomyocytes are therefore an amenable cellular tool for in vitro apoptosis assays investigating the activation of caspase pathways.
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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CaspaseApoptosisApoptosis, or programmed cell death, is a vital process for healthy tissues and is key to our understanding of disease mechanisms like cancer and neurodegeneration. Research tools enable apoptosis detection, quantification, and analysis. Browse products for apoptosis research in our peer-reviewed product directory.Cardiomyocytes