Hypoxic Cell Culture in the New Brunswick™ Galaxy® 170R Incubator: Normal Growth, Morphological Changes

13 Feb 2015

Brunswick Galaxy 170 series incubators have a wide array of options that allow for not only CO2 and temperature control, but also for O2 concentration control which can be used to create a hypoxic internal environment. To demonstrate the low O2 capability of this system, prostate cancer cells (LNCaP) were grown in 2 % O2 and their growth and morphology were observed. In contrast to the effects of hypoxia on normal cells, LNCaP cells were able to grow normally and displayed morphological changes.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.IncubatorsLaboratory incubators are used to provide a stable environment to cultivate and store samples in, giving you optimal control of temperature, humidity, oxygen and CO<sub>2</sub> levels. Incubators are necessary equipment for cell culture and microbial research. Control temperature with heated or refrigerated incubators, or the levels of CO<sub>2</sub> and oxygen with CO<sub>2</sub> incubators. Using environmental chambers, create a humid climate for testing samples. Find the best incubators in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HypoxiaProstate Cancer
Hypoxic Cell Culture in the New Brunswick™ Galaxy® 170R Incubator: Normal Growth, Morphological Changes