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HPLC method to differentiate four THC stereoisomers formed from Δ9-THC degradation

Cayman Chemical Co.
29 Jul 2021

Degradation of Δ9-THC to Δ10-THC and Δ6a,10a-THC isomers, provides a challenging separation in cannabis-derived products. In this application note, learn how the use of an immobilized cellulose chiral column under normal-phase liquid chromatography conditions provides an analytical method to fully separate these four THC isomers for identification and accurate determination of potency.

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Phytocannabinoid Mixture 11 (CRM) (1 ml, 250 µg/ml)

Cayman Chemical Co.

Phytocannabinoid Mixture 11 (CRM) (1 ml,  250 µg/ml) (Item No. 21306) is a certified reference material mixture provided as a DEA exempt preparation. Included are Δ9-THC, Δ8-THC, tetrahydrocannabinolic acid A, cannabinol, cannabidiol, cannabidiolic acid, (±)-cannabichromene, cannabigerol, cannabigerolic acid, cannabidivarin, and tetrahydrocannabivarin. It is supplied in an amber ampule in which the headspace has been purged with argon.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CannabinoidsCannabinoids are compounds found in cannabis that have therapeutic effects, such as pain relief, anti-inflammatory properties, and potential neuroprotective benefits. Research into cannabinoids is growing, particularly in the fields of pain management, neurological diseases, and cancer treatments. Browse our peer-reviewed product directory to find the best cannabinoid research tools, compare products, check reviews, and get pricing directly from manufacturers.Cannabis

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HPLC method to differentiate four THC stereoisomers formed from Δ9-THC degradation