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HPLC challenges and solutions: A guide to improved HPLC routines
9 May 2025Whether you are looking for assistance with your daily HPLC routines, confidence in your HPLC results, protecting your HPLC investments, or supporting your sustainability goals, Agilent products can help support your lab's HPLC goals for both today and tomorrow. In this application note, Agilent Technologies demonstrates how your lab can benefit from its solutions to HPLC challenges.
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Agilent Analytical HPLC Systems
Agilent TechnologiesWhether you need assistance with your daily HPLC routines, confidence in your HPLC results, protection for your HPLC investments, or support for your sustainability goals, Agilent HPLC & UHPLC products can help achieve your lab's HPLC objectives both today and tomorrow.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.Liquid ChromatographySustainabilitySustainability in research and industry focuses on reducing environmental impact, conserving resources, and promoting long-term ecological balance. Innovations in sustainable practices include renewable energy, waste reduction, and eco-friendly materials. Explore more sustainabile solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.