How to Use Corning 3D Spheroid-Qualified Primary Human Hepatocytes

7 Dec 2018

This application note describes how 3D spheroid-qualified primary human hepatocytes (PHHs) can be used to create 3D PHH spheroids for high throughput screenings or investigative studies requiring long-term culture, e.g. chronic hepatoxicity assays.

Corning® 96-well Black/Clear Round Bottom Ultra-Low Attachment Surface Spheroid Microplate, Bulk Packed, with Lid, Sterile, 10/Bag

Corning Life Sciences

Ultra-low attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 300 µL total volume Recommended working volumes of 0.075 to 0.30 mL Sterilized by gamma radiation Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.Hepatocytes
How to Use Corning 3D Spheroid-Qualified Primary Human Hepatocytes