How to start a SPRi experiment
11 Jan 2018In the following application note, Horiba addresses several questions to consider before conducting a surface plasmon resonance imaging experiment. For example, multiple biochip sensors can be used and a number of functionalized surface chemistries are available to directly immobilize ligands of interest. Controls are another important consideration as refractive index changes and non-specific interactions are subtracted from signals. Running buffers can improve specific interactions and samples must be carefully prepared to avoid refractive index changes. Furthermore, the capacity for ligand regeneration is discussed, as well as how to evaluate the data produced by SPRi software.
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OpenPlex - Flexible Surface Plasmon Resonance Imaging System
HORIBA ScientificDesigned to meet the demands of biologists, biochemists and biophysicists, the OpenPlex is a flexible surface plasmon resonance imaging system. OpenPlex is your companion for the development of label-free and multiplexed bio-assays and molecule detection. It is a robust and compact system designed for simple use and high versatility. Its open format, dedicated sensor chips and manual operation enable numerous types of experiments to be explored without compromise, covering chemistry, biochemistry, physico-chemistry and biomolecular interactions. Open your research areas with OpenPlex! Choose between three different flow cell configurations without concession: • Normal flow cell for standard surface plasmon resonance imaging experiments • Window flow cell, compatible with fluorescence measurements • Cuvette cell, compatible with electrochemistry measurements