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How to Separate Drugs and Metabolites

25 Oct 2018

This application note explains a drug and metabolite separation model, how to choose the right column for your separations, and sample preparation solutions.

Luna Omega PS C18

Phenomenex Inc

Unique, 100 % aqueous stable mixed-mode phase that provides both polar and non-polar retention. The surface contains a positive charged ligand which aids in the retention of acidic compounds through ionic interactions, while the C18 ligand promotes general reversed phase retention. The positively charged surface also improves basic compound peaks shape through ionic repulsion. Available in: 1.6µm, 3µm, and 5µm

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationHPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.
How to Separate Drugs and Metabolites