ResourceLife Sciences
How to Purify a Membrane Protein
2 Oct 2011Different tissues, organisms and membrane types sometimes require different detergents and conditions for purification; e.g., bacterial sources may require distinct solubilization procedures from mammalian sources. This application note outlines some of the methods used in purifying membrane proteins. Some of the properties of a good detergent are also described.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Detergent