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How to Perform High Throughput siRNA Transfections with the CyBi®-Well Vario Workstation

Analytik Jena GmbH+Co. KG
22 Oct 2014

In the field of gene silencing, RNA molecules of 21-28 base pairs in length are used for the inhibition of gene expression in mammalian cells because long dsRNA molecules induce the mammalian interferon response. These molecules are termed small interfering RNA (siRNA). One effective method for delivery of siRNA into cells is transfection through lipofection. This method is believed to be especially effective in the cell growth phase. This is achieved by forming a transfection complex of siRNA and a cationic lipid-based transfection reagent which is then incubated with cells to deliver the siRNA molecule into the cytoplasm. This poster demonstrates the suitability of the CyBi®-Well Workstation for automated dispensing of samples for siRNA lipofection.

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CyBio Well Vario

Analytik Jena GmbH+Co. KG

The CyBio Well Vario is CyBio´s automated, simultaneous pipetting platform which can be equipped with 11 interchangeable pipetting heads to offer highest pipetting precision and flexibility in a compact design. A large variety of disposable tips, highly precise ceramic tips or glass capillaries allow liquid transfers in a broad volume range of 4 orders of magnitude (25 nl up to 250 µl) on one pipetting system. 14 different capillary magazines are available for CyBio´s Capillary Head, which is especially designed for precise low-volume compound transfers (25—1000 nl). The CyBio Well vario is compatible with many specific accessories and can be adapted to every screening environment to create powerful liquid handling platforms for today and tomorrow. Optional stacker or dispenser integration together with the involvement of versatile peripheral devices can be easily implemented. Challenging liquid handling applications like working in high density microplate formats or in HTS environments can be easily achieved. CyBio´s outstanding customer service and application support is a well appreciated inherent part of every CyBio Well vario system. CyBio Well Vario Features: Extremely flexible through enormous volume spectrum Highest precision in the whole volume range Unique pipetting technology prevents cross contamination Easy application set-up using CyBio Composer software Saves reagent costs with accurate low volume pipetting

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Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.siRNATransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.

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How to Perform High Throughput siRNA Transfections with the CyBi®-Well Vario Workstation