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How to Get the Most Accurate and Reliable Data from HPLC using Ultrapure Water
12 Nov 2018As a scientist, getting reliable, accurate, and consistent data from your HPLC instrumentation is critical. Unreliable data means wasted time and effort on your part. It has been estimated that most difficulties with HPLC are a result of column problems due to poor quality lab water. Water that’s not purified to the highest standards can lead to ghost peaks, blocked chromatography columns, poor separations, and more.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Water PurificationThere are a number of water purification methods available to remove contamination from laboratory water. These systems include demineralization, reverse osmosis, filtration and ultrafiltration. A combination of methods is sometimes necessary to achieve purified water suitable for use in experiments.Ultrapure WaterUltrapure water is the highest purity water and contains the lowest levels of organic and inorganic contaminants, particulates and microorganisms.Data AcquisitionData acquisition refers to the collection and organization of information and data sets.Pure WaterPure water is processed to remove significant level of contaminants and is suitable for use in many laboratory applications.