ResourceSeparations
How to Avoid Formation of Oils and Gums When Drying HPLC Fractions
2 Oct 2017Traditional concentration of reverse phase HPLC fractions to dryness in a Genevac HT evaporator is a fast and safe process. However, for certain compounds a fully dried result can be hard to achieve due to compound interactions with water boosting up the boiling point making drying difficult. Some compounds also tend to naturally form a gum or oil. In such cases, subsequent characterization by NMR normally indicates the presence of residual solvent, whilst samples which are not completely dry are of little use in biological assays since accurate weights cannot be obtained.This application note presents the Lyospeed™ method for avoiding these problems.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Evaporators and Freeze DryersCentrifugal evaporation is used for solvent removal from a sample. This is a useful technique for sample concentration or sample drying. Many centrifugal evaporators are modular with a central vacuum pump for generating low pressure. Useful features of evaporators or vacuum ovens include minimal solvent bumping, solvent resistance, computer control, temperature regulation and benchtop design.LyophilizationHPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.

