ResourceLife Sciences
How the choice of assay and system influences results
30 Apr 2024Since its introduction in 2002, free light chains (FLC) testing has become crucial for diagnosing and managing monoclonal gammopathies. Recommendations from the International Myeloma Working Group (IMWG) and other societies rely heavily on serum free light chains (sFLC), particularly the involved/uninvolved FLC ratio, to indicate different stages of these disorders. Multiple assays and detection methodologies exist, with nephelometry known for its sensitivity advantage over turbidimetry. Siemens Healthineers highlights how the choice of assay and system significantly influences results, as shown by studies comparing polyclonal FREELITE® assays and monoclonal N Latex FLC assays, revealing variations in sensitivity and performance.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Free Light Chains