ResourceLife Sciences
How Can Multiplexing Speed up the Optimization of your SPRi Experiment? Example for an Antibody Biochip
11 Jan 2018In this application note, Horiba describes the multiplexing capacity of a surface plasmon resonance imaging biochip loaded into the XeIPIeX instrument. XeIPIeX enables multiple label-free molecular interactions to be monitored in parallel. This technology is demonstrated here for the optimization of antibody immobilization on a SPRI-BiochipTM.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Antibody ArraysSurface Plasmon ResonanceMultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.