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Host Cell Protein Removal with Sartobind STIC® PA Pico

Sartorius Group
20 Jun 2016

This application note details a method for host cell protein removal using the Sartobind STIC® PA pico from Sartorius. Sartobind® STIC (Salt Tolerant Interaction Chromatography), is a membrane concept based on weak anion exchange chemistry that was developed to facilitate a wider design space for FT-AIEX membrane chromatography at commercial scale. Sartobind STIC PA is able to overcome the limitations of HCP removal at high conductivities, allowing for >10 times reduced sample consumption and substantial cost savings for virus validation studies.

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Sartobind STIC® salt-tolerant membrane chromatography

Sartorius Group

Disposable capsules with salt tolerant interaction membranes for polishing in biomanufacturing. The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines. Plug and play device Can be used like a filter Enhanced flexibility Reduces validation costs Recommended flow rate of up to 30 bed volumes/min

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Antibody PurificationProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.

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