Homogeneous Add-Mix-Read Assay for Total and Oxidized Glutathione
27 Aug 2015A wide variety of compounds can induce intracellular formation of reactive oxygen species (ROS) and the resulting cellular damage can eventually lead to apoptosis or necrosis. Measuring the ratio of reduced to oxidized glutathione (GSH/GSSG ratio) is a way to determine how such compounds alter the redox potential in the cell. GSH/GSSG-Glo allows for rapid, direct measurement of total and oxidized glutathione in cell-culture wells using low cell density.
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GSH-Glo™ Glutathione Assay
Promega Corp.The GSH-Glo™ Glutathione Assay is a luminescence-based assay for the detection and quantification of glutathione (GSH). The assay is based on the conversion of a luciferin derivative into luciferin in the presence of glutathione, catalyzed by glutathione S-transferase (GST). The signal generated in a coupled reaction with firefly luciferase is proportional to the amount of glutathione present in the sample. Luminescence is generated in less than one hour. The assay generates a stable luminescence signal and is simple, fast and easily adaptable to multiwell formats such as 96- and 384-well plates. This assay can be used for detection and quantification of GSH in cultured cells or in various biological samples.