Highly-efficient miRNA Isolation using the Agencourt FormaPure and RNAdvance Cell v2 Kits and Biomek Automated Extraction Methods

22 May 2013

This application note describes an efficient and robust miRNA extraction method using Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation. The SPRI procedure is an easy, rapid, high yield, and automation-friendly nucleic acid purification method that does not require organic solvents, centrifugation or filtration steps. This method uses carboxyl-coated magnetic particles that reversibly bind nucleic acids in the presence of binding buffers and crowding reagents.

RNAdvance Cell v2 kit

Beckman Coulter Life Sciences

The Agencourt RNAdvance Cell v2 kit is based on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead technology, known for its ability to consistently deliver pure nucleic acids of the highest quality and yield.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.miRNA