Highly Efficient Delivery of Viral RNA Genomes Using a Lipid-Based Transfection Reagent Compared to Electroporation

9 Feb 2015

To optimize and facilitate the introduction of viral RNAs into mammalian cells, this application note compare lipid-based transfection reagents and electroporation for their ability to deliver hepatitis C virus (HCV) and yellow fever virus replicons to HuH7 cells.

TransIT®-mRNA Transfection Kit

Mirus Bio LLC

A high efficiency, low toxicity, large RNA transfection reagent for mammalian cells.TransIT®-mRNA Transfection Kit is designed to transfect RNA into a broad range of cell types with minimal cellular toxicity. RNA delivery avoids transcriptional regulation effects by directly delivering the RNA to the cytoplasm for expression. The TransIT-mRNA Transfection Kit can be used to deliver a variety of RNA molecules including mRNAs and viral RNAs (2–10 kb). It can be used for multiple applications such as short-term protein expression, viral production and replication studies.TransIT®-mRNA Transfection Kit Features:•Low Cellular Toxicity - Maintain cell density and reduce experimental biases.•High Efficiency Delivery - Achieve RNA delivery in a large population of cells to ensure experimental success.•Serum Compatible - Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health.•Deliver Various Sizes of RNA - Ideal for specialized applications, such as viral production and protein expression from mRNA.

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Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.Electroporation
Highly Efficient Delivery of Viral RNA Genomes Using a Lipid-Based Transfection Reagent Compared to Electroporation