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Highly Efficient ChIP qPCR Assay Kit for FFPE Samples

Porvair Sciences Ltd

Highly Efficient ChIP qPCR Assay Kit for FFPE Samples

15 Sept 2015

In this application note, Chromatrap® introduces a new Formaldehyde Fixed Paraffin-Embedded (FFPE) ChIP-qPCR Kit which overcomes the widely acknowledged shortfalls of the technique. Available in 96-well or spin column formats the new Chromatrap® FFPE ChIP-qPCR kit not only provides greater flexibility and more IPs per sample but is also proven to work well across a range of human and animal FFPE samples. Requiring 10x less starting material compared to traditional methods, the Chromatrap® FFPE ChIP-qPCR kit produces 4x better pull down than even the best competitive kits. The new Chromatrap® FFPE ChIP kit works equally well with high or low abundant marks and provides valuable data where the transcription factor loci are already identified.

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Chromatrap® FFPE ChIP Kit

Chromatrap

Epigenetic identification and targeting in clinical FFPE tissues is a crucial step for potential epigenetic therapeutics and personalized medicine therapies. FFPE is a standard method to archive and preserve medical tissue biopsy samples. These archives, kept in many hospitals world-wide, are a huge, rich and untapped sample resource for genetic research. Due to the cross-linking of proteins by formaldehyde, FFPE tissues present a particular challenge for ChIP analysis. In addition, the small size and delicate nature of the tissue, and the difficulty in extracting samples from paraffin, used to provide tissue support after preservation, can damage complex cellular complexes such as chromatin. ChIP assays from these samples have therefore proved time consuming and difficult.  Chromatrap® FFPE ChIP kit overcomes these issues and provides the following benefits: Provides greater flexibility and more IPs per sample Works across a range of FFPE samples, human and animal Available in 96-well plate or spin column format Works equally well with high and low abundant marks Provides sufficient DNA to perform library preps for ChIP-seq assays

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Animal ModelsThe use of non-human animals in experiments or behavorial observations. The research is conducted inside universities, medical schools, pharmaceutical companies, farms, defence establishments, and commercial facilities that provide animal-testing services to industry. It includes pure research such as genetics, developmental biology, behavioral studies, as well as applied research such as pharmaceutical testing in pre-clinical, before human, studies. ChIP-SequencingqPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.TranscriptomicsFFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Highly Efficient ChIP qPCR Assay Kit for FFPE Samples