High Yields of Transfected Cells with BRANDplates® cellGrade™ Premium Surface

5 Dec 2014

Transfection is defined as non-viral DNA/gene delivery into eukaryotic cells performed by several chemical, physical or biological methods. Serum-free culture medium is necessary for successful transfections to avoid nucleases degrading or forming complexes with DNA. This application note compares three different microplate surfaces regarding their ability to support proliferation and attachment of transfected cells during washing steps.

BRANDplates Non-treated 96, 384, 1536-well Microplates

BRANDTECH® Scientific, Inc.

BRAND 96, 384, 1536-well polystyrene and polypropylene microtitration plates are excellent for cell and tissue culture, sample storage, immunological assays, and other applications. BrandTech Plates have 96, 384 and 1536 wells and are available with 330µL or 400µL well volumes. They are fully compatible with most standard plate readers and washers. Both sterile and non-sterile BRAND plates are available with rounded, V-shaped, or flat well bottoms to meet the needs of most applications.Sterile, treated plates with hydrophilic surfaces are also available. Design features of the BRAND 96, 384 and 1536-well microplates help reduce evaporation and contamination. Raised rings around wells simplify sealing with adhesive films and mats. Recessed hollows between wells reduce oversplash. Optional lids are equipped with condensation rings.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.Microplate Washer