ResourceLife Sciences
High-throughput Human Serum and Immunoglobulin G N-Glycome profiling with the AdvanceBio Gly-X InstantPC Technology for biomarker discovery
7 Apr 2022In this application note, a method for high-throughput glycome profiling is described. Protein glycosylation is an intracellular process resulting in a glycan profile that is dependent upon the host cell and the translated protein. This workflow enables high-throughput efficiency and reproducibility in sample preparation for obtaining high-sensitivity N-glycome profiling and holds great potential for the discovery of disease diagnostic biomarkers.
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AdvanceBio Gly-X Technology
Agilent TechnologiesThe Agilent AdvanceBio Gly-X (formerly ProZyme) technology is a new generation of N-glycan sample preparation
InstantPC Labeled Glycans
Agilent TechnologiesAgilent InstantPC (formerly ProZyme) labeled glycan standards and libraries are commonly used as qualitative standards for LC/FLD and LC/MS.
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GlycobiologyGlycobiology encompasses research into the biological functions, synthesis and structures of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides. Mass spectrometers and their software are commonly used in the study of glycobiology, along with structure predicting tools, chromatography and NMR.GlycansGlycosylationGlycoproteins