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High-throughput DNA library preparation for nanopore sequencing using DreamPep NGS and ligation sequencing KIT XL v14

Tecan

Scalable DNA library preparation protocol for Oxford Nanopore sequencing devices

5 Apr 2023

In this application note, Oxford Nanopore Technologies describes how it has developed a sequencing protocol for the analysis of longer DNA fragments. This approach provides comprehensive genomic insights, including the detection of structural variants, copy number variations, and methylation patterns. Obtaining complete genome information is essential for understanding chromosome function, human diseases, and genomic variation. The integration of the Ligation Sequencing Kit XL V14 on the DreamPrep NGS workstation promises a reliable and automated solution for high-throughput, long-read DNA library preparation. The resulting libraries demonstrate comparable quality to those produced manually and are compatible with Oxford Nanophore Technology sequencing devices sequencing devices.

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DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNA MethylationGenome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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High-throughput DNA library preparation for nanopore sequencing using DreamPep NGS and ligation sequencing KIT XL v14