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High Throughput Cell Adhesion: Screening of VLA-4 Antagonists Under Physiological Shear Flow Conditions

Fluxion Biosciences
13 May 2013

Cell adhesion governs countless biological phenomena in health and disease. This application note presents a screen of three monoclonal antibodies directed against very late antigen-4 (VLA-4) for assessment of adhesion reduction to vascular cell adhesion molecule -1 (VCAM-1) in the Bioflux™ System. This screen analyzed the number of cells adhering to the VCAM-1 substrate, as well as the morphological characteristics of attached cells. The time to perform the entire experiment from coating the plate with VCAM-1 to manual data collection is reduced to 3 hours in comparison to 6-18 hours for a standard microplate adhesion inhibition assay.

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A Complete Solution for Functional Live Cell Assays Under Controlled Shear Flow—Great for Labs With an Existing Inverted Microscope

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Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell-Based PlatformInhibitorsMonoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cell Migration

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High Throughput Cell Adhesion: Screening of VLA-4 Antagonists Under Physiological Shear Flow Conditions