ResourceSeparations

High-Speed, High-Resolution Analysis of Low Molecular Weight Polymers Using the Advanced Polymer Chromatography (APC) System

25 Nov 2014

In this application note, separations using the ACQUITY APC System are compared to conventional GPC separations. Faster analysis, improved resolution, and the beneficial effect on calibration of low molecular weight oligomers using a low-dispersion system with sub 3-μm hybrid particle technology columns is illustrated. The combination of these technologies allows more robust and precise determination of molecular weight parameters for low molecular weight polymer samples.

ACQUITY Advanced Polymer Chromatography System

Waters

Developing differentiated polymers and deploying a sustainable operation are a key focus for the polymer and plastics industries. Current GPC/SEC methods are limited by a lack of resolution, separation times, and the volume of solvent consumed per analysis. The ACQUITY Advanced Polymer Chromatography (APC™) System is a breakthrough technology that defines the ultimate in size-based chromatographic separations, delivering more information about your polymers faster than ever before. This means better characterization, improved asset utilization and a superior solution for achieving corporate innovation and sustainability goals. Key Features: New Column Technology – ACQUITY APC™ Columns utilize small, high pore volume bridged-ethyl hybrid particles that provide significant gains in stability, flexibility and faster separations. Stable Refractive Index Detection – The ACQUITY Refractive Index (RI) Detector is optimized for low dispersion, but with the low noise and drift performance required for accurate integration, even at low polymer concentrations. Precise Solvent Management - Precise flow delivery of the isocratic solvent manager ensures calibrated system provides accurate molecular weight data, day after day. Advanced Column Heating Module - Ensures highly repeatable thermal environment for banks of ACQUITY APC Columns. Empower® 3 Software with GPC option – Quickly and easily review, compare, and report polymer analysis data. Key Benefits Polymer Characterization - Provides unparalleled polymer peak resolution, particularly for low molecular weight oligomers. Speed of Analysis - Obtain accurate and repeatable polymer molecular weight information up to 5 to 20 times faster than traditional GPC/SEC methods. Cost Reduction - Lower analysis cost by reducing solvent consumption and waste disposal volumes. System Flexibility - Run diverse polymer applications on a single system.

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Empower™ Chromatography Data System

Waters

The Empower Chromatography Data System is Waters’ compliance-ready chromatography data software (CDS) for advanced data acquisition, processing, and reporting that simplifies the management of chromatography test results.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Molecular Weight AnalysisMolecular weight analysis determines the size and structure of biomolecules, essential in protein characterization, polymer studies, and drug development. Techniques like mass spectrometry ensure precise results. Compare solutions for molecular weight analysis in our peer-reviewed product directory.SECSize exclusion chromatography (SEC) separates molecules based on size, ideal for protein purification, polymer analysis, and biopharma applications. SEC ensures precise characterization of biomolecules for research and industry. Explore peer-reviewed SEC products and compare options for your lab.PolymersPolymers are large molecules made up of many repeat units. Natural polymers such as DNA and proteins are essential to life, whereas synthetic polymers such as polystyrene and polyethylene are used widely due to their functionality. Typical techniques for analysis include GPC, SEC, DSC, FT-IR and NMR.
High-Speed, High-Resolution Analysis of Low Molecular Weight Polymers Using the Advanced Polymer Chromatography (APC) System