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High-Resolution Cation-Exchange Alternative to Peptide Mapping for Protein ID and QA/QC
13 Jan 2015Although a reversed-phase separation is the typical choice for separating peptides, high-resolution ion-exchange chromatography is an alternate method that provides additional information and a different selectivity. The work shown here demonstrates that strong cation-exchange chromatography can provide good resolution for peptide mapping, and thereby serve as an alternate or supplementary method for separating peptides.
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Peptide Mapping