High-Performance Vion IMS QToF for Monoclonal Antibody Intact and Subunit Mass Analysis

23 Mar 2017

High-resolution mass spectrometry is commonly used for assessment of the product quality and safety of biotherapeutic protein products. In this application note, Vion IMS QToF Mass Spectrophotometer was integrated with a compliant-ready informatics tool, UNIFI Scientific Information System, for intact mAb and subunit mass analysis. This system delivers accurate and reproducible mass measurements at the intact and subunit levels and enables confident identification and robust quantification of the protein modifications.

Vion IMS QTof

Waters

Vion IMS QTof is a high resolution QTof mass spectrometer that delivers the selectivity of ion mobility to every scientist, for every analysis. Does high resolution, accurate mass data always lead to the correct answer first time? Avoid additional confirmatory experiments by utilizing the selectivity of ion mobility. Featuring new geometry and including XS Ion Optics and the QuanTof2 detection system, the Vion IMS QTof  has the sensitivity and dynamic range to make ion mobility quantitative and routinely usable, enabling: Easier data interpretation as ion mobility cleans up and simplifies every spectrum Confident identification and quantification of analytes Faster method development and higher sample throughput Greater selectivity than conventional MS/MS with HDMSE With Vion IMS QTof you can use the selectivity of ion mobility to quickly refine spectra and discount chromatographically co-eluting compounds and background interferences. Enabling you to characterize your compounds with confidence. The ability to gather and process CCS data for every ion gives you more confidence in your data than ever before. Vion IMS QTof is driven by UNIFI scientific information system to deliver the most comprehensive analytical workflow, enabling you to target, identify, and review results in a single software platform. Make more informed decisions, faster. CCS provides chromatographic retention time independent data points, enabling confident identification of your analytes and reducing the risk of false positives and false negatives. Even during initial sample analysis, without any retention time knowledge, you can use theoretical exact mass and CCS library values to identify unknown and unexpected components. Applications: Screening - Meeting legislative and regulatory demands requires the utmost confidence in your data. UNIFI informatics workflows integrate the value of CCS, reducing false positives and false negatives driving laboratory efficiency. Pharma - The combination of UNIFI and Vion IMS QTof brings ion mobility to your drug discovery experiments while maintaing ease and efficiency you need for your routine Met ID processes. Omics - A recurring challenge for omics studies is the identification and structural elucidation of metabolites, lipids, and peptides of interest. Incorporating Vion IMS QTof into your workflow gives you not only CSS data from every component, but also increase analytical space and resolution.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Chem / BioinformaticsCheminformatics and bioinformatics are computational techniques used in chemistry and biology, respectively, for data acquisition, processing and storage. Cheminformatics focuses on compound information, whereas bioinformatics is mainly applied to analysis and modeling of genomics, genetic and sequencing information. Hardware and software is available for data acquisition, analysis, management and storage.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Therapeutic AntibodiesTherapeutic antibodies are antibodies engineered for use in treating diseases. They can be designed to target specific antigens, blocking harmful interactions or modulating immune responses. Therapeutic antibodies are critical in treating conditions like cancer, autoimmune disorders, and infectious diseases. Explore the best therapeutic antibody products in our peer-reviewed product directory; compare options, check reviews, and get pricing directly from manufacturers.BiotherapeuticsBiotherapeutics are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or in <i>in vivo</i> diagnostics. The most well-known example of a biotherapeutic product, and the first to be approved for therapeutic use, was recombinant human insulin.Drug DevelopmentDrug development refers to the process of bringing a new drug to market.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.
High-Performance Vion IMS QToF for Monoclonal Antibody Intact and Subunit Mass Analysis