High Efficiency Transfection of iCell® Cardiomyocytes and Stem Cell Relevant Cell Sources

31 Oct 2014

Transfection of nucleic acids such as plasmid DNA, mRNA and siRNA serves as a vital tool in stem cell reprogramming and differentiation processes. This poster demonstrates how TransIT® Transfection Reagents and Ingenio® Electroporation Solution offer efficiency delivery of plasmid DNA and siRNA to iCell Cardiomyocytes and induced pluripotent stem cells.

Ingenio® Electroporation Solution

Mirus Bio LLC

High efficiency electroporation using any instrumentIngenio® Electroporation Kits and Solution provide a universal, high efficiency, low toxicity solution for electroporation of DNA or siRNA into hard to transfect cell types. Electroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Electroporation Solution Features:•High efficiency electroporation of hard to transfect cell lines and primary cells•Compatible with most conventional electroporation devices including Lonza-Amaxa®, Bio-Rad® or Harvard BTX®•Ideal for plasmid or siRNA electroporation

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TransIT®-2020 Transfection Reagent

Mirus Bio LLC

A high performance, animal-free, broad spectrum DNA transfection reagentTransIT®-2020 Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells. TransIT-2020 affords high levels of gene expression in typically hard-to-transfect cell types, including primary cells and is suitable for both transient and stable transfection.TransIT-2020 is composed of animal-origin free components and is serum compatible, eliminating the need for any culture medium change after transfection.TransIT®-2020 Transfection Reagent•Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cell lines and primary cells•Gentle to Your Cells - Balances high efficiency nucleic acid delivery and low cellular toxicity, imperative to achieving reliable experimental results•Superior Transfection of Insect Cells - Obtain higher expression than other insect cell transfection reagents•Animal Origin Free - TransIT®-2020 provides high performance with maximum compatibility

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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.Stem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine. Cardiomyocytes
High Efficiency Transfection of iCell® Cardiomyocytes and Stem Cell Relevant Cell Sources