High Content Duraclone Reagents on the CytoFLEX Flow Cytometer: Instrument Setup Using the CytoFLEX Compensation Library

This application note explains the set-up procedure for use of a new proprietary dry reagent formulation that secures long-term fluorochrome stability while stored at room temperature with the CytoFLEX flow cytometer. Tandem dyes play an important role in high content flow cytometry. However, degradation of tandem dyes over time due to light and oxygen exposure as well as inappropriate storage conditions can severely affect their emission spectra and may result in the need for frequent compensation adjustment. Furthermore, manufacturer dependent spectral variability may occur in tandem dyes that could require lot-specific compensation settings.