ResourceLife Sciences
High Content Analysis of Cardiac Differentiation from iPS Cells
21 Jun 2011In this application note, Molecular Devices use iPS-derived cardiomyocytes as a model system for testing toxicity and cardioprotection effects of drug candidates. High content imaging methods are described that allow automatic evaluation of differentiation of iPS derived cardiomyocytes. The model system is used to determine IC50s of several cardiotoxic drugs (doxozamine, imatinib, mitomycinA, verapamil). In addition, the cytoprotection effect of sphingosine phosphate against cytotoxic damage is analysed.
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. 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