ResourceLife Sciences
High Cell Density Fermentation of <i>Escherichia coli</i> Using the New Brunswick™ BioFlo® 115
18 Feb 2015This application note presents a successful example of a high density fermentation of Escherichia coli (E. coli) using the New Brunswick BioFlo 115 benchtop, autoclavable fermentor from Eppendorf. The highest optical density (OD600) achieved in this study was 140 at 11 h without optimized fermentation medium and conditions.
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MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.Escherichia coli