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High-Cell-Density Cultivation of Escherichia coli in a BIOSTAT® D-DCU 10-3 Stainless Steel Bioreactor

Sartorius Group
29 Oct 2014

Generally, stainless steel bioreactor systems are used for microbial fermentations because their design principles are well known. Consequently, there is a lot of experience and knowledge for these bioreactors resulting in their use over several decades. Furthermore, such bioreactors are available in a scalable range from laboratory to production scale, which enables easy process transfer and scale-up. BIOSTAT® D-DCU is a bioreactor for both microbial and cell culture applications, offering the capability of sterilization in place (SIP). This application note demonstrates the high-cell-density cultivation of Escherichia coli in a BIOSTAT® D-DCU 10-3 Bioreactor.

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BIOSTAT® D-DCU

Sartorius Group

The BIOSTAT® D-DCU is a compact, bioprocess system available for microbial and cell culture applications with working volumes from 10 to 200L. The BIOSTAT D-DCU incorporates many desirable and advanced features to fulfil virtually any demand for modern bioprocess application, such as: Automatic Sterilization in Place (SIP) and Cleaning in Place (CIP), Dual pH and DO measurement capability, Lid lifting device, WIT-Ready filter housings, Dual exhaust filter housing line, Tube and shell exhaust cooler, exhaust heater, automatic or manual addition arrays as well as other accessories. Furthermore the BIOSTAT D-DCU is designed to interface Single use storage bags for media addition and harvest as well as single use sampling systems. Due to the modular approach, resulting in various configurations; from baseline to fully-featured, the system offers an excellent solution for every budget and every need.Features: Single or Twin configuration 10L, 20L, 30L, 50L, 100L, and 200L working volume For microbial or cell culture applications Automated Sterilization in Place (SIP) Automated Cleaning In Place (CIP) Space saving design Full qualification support

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Scale-upScale-Up is generally defined as the process of transferring the results of the discovery phases of the drug discovery process, obtained on a laboratory scale, to the pilot plant and finally to production scale. Scale-Up uses reactors and pump systems as well as reagents, standards and buffers. Often Scale-Up is provided as a service by Contract Manufacturing Organizations (CMOs). Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.Process DevelopmentProcess development aims to optimize the performance of manufacturing systems. This involves the assessment of quality and efficiency of both processes and products. This is particularly important in the scale-up of chemical processes.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.

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