Handling of Adherent Cells with the CyBi®-Drop 3D

In cellular assays, the dispensing of assay reagents or media into the wells of an assay plate with an adherent cell monolayer on the well bottom is often required. The complete preservation of the cell monolayer is an essential precondition to ensure the correct evaluation of the assay results. The CyBi®-Drop 3D offers fast and accurate non-contact dispensing of up to four reagents to microplates. Especially designed dispensing combs with 8 or 16 channels made from stainless steel or PEEK are available for different applications. CyBio® Software enables the flexible adjustment of the pump speed, the dispensing volume per stroke and the stroke number per well. In this application note, the optimal dispensing conditions were defined which prevented the disruption of a CHO cell monolayer in 96 well microplates via optimizing these parameters and subsequent stereo microscopic analysis.