ResourceLife Sciences
Guidelines for protein purification at low temperature
11 Oct 2023Protein purification at low temperatures is crucial for preserving protein integrity and preventing proteolytic degradation, as well as managing protein aggregation tendencies. This application note offers general principles for successful protein purification in cold environments, with ÄKTA™ systems, chromatography resins, and prepacked columns typically performing well at 4°C to 8°C, similar to room temperature results.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.