Guide to novel approaches in spatial biology: Gain deeper insights into tissue heterogeneity

16 Jan 2023

Spatial biology techniques are expanding our understanding of biological architecture with ever greater resolution in the context of tissue microenvironment. Next-generation molecular profiling solutions for the analysis of spatial gene expression look set to be game-changers, allowing us, for example, to classify tissue based on total mRNA, and enabling advances in spatially resolved gene expression that tell us where in a cell that expression is changing.

In this eBook, learn how coupling single-cell approaches with new spatial analyses of gene expression is enabling researchers to see biology in new ways through significant shifts in spatial resolution and scale, whether for cancer cell profiling or greater basic understanding in neuroscience.

Download the eBook to learn how the latest spatial biology profiling platforms from 10x Genomics can be used to resolve cancer tissue types and brain architecture in normal and diseased states, including case studies of the following tissue types:

  • FFPE prostate cancer tumor samples
  • Triple negative breast cancer
  • Human squamous cell carcinoma
  • Brain architecture
  • Alzheimer’s disease markers

Visium Spatial Gene Expression

10x Genomics

Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh-frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Guide to novel approaches in spatial biology: Gain deeper insights into tissue heterogeneity