ResourceSpectroscopy
Glycoform Profiling from Therapeutic Antibodies at the Protein, Peptide and Cleaved Glycan Level using Mass Spectrometry
18 Aug 2015The glycans (oligosaccharides) attached to proteins play a crucial role in many recognition, signaling, and adhesion events within and between cells. The carbohydrate composition and the structure of the glycans are important for the safety and efficacy of these therapeutic proteins. Understanding exactly how the carbohydrates in therapeutic proteins modulate their functions in patients is key to the development of an optimal glycan profile, emphasizing the importance of performing glycan analysis. This application note summarizes the glycoform profiling data from a therapeutic antibody at three different levels: intact protein, peptide (glycopeptides) and cleaved glycans using mass spectrometry (MS).
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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
GlycansProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Therapeutic AntibodiesTherapeutic antibodies are antibodies engineered for use in treating diseases. They can be designed to target specific antigens, blocking harmful interactions or modulating immune responses. Therapeutic antibodies are critical in treating conditions like cancer, autoimmune disorders, and infectious diseases. Explore the best therapeutic antibody products in our peer-reviewed product directory; compare options, check reviews, and get pricing directly from manufacturers.Glycoproteins