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Getting High Yields of gDNA from Plant Samples; MACHEREY-NAGEL and Tecan

Tecan
14 Jul 2015

Plant research is often geared towards crop improvement, and focuses on yield and robustness to pathogens and other stress factors, such as heat or draught. It is often necessary to sequence the genome, creating the need for purification of high quantities of gDNA. MACHEREY-NAGEL has developed the NucleoSpin Plant II Midi kit to meet the demand for efficient purification of high quality DNA from a variety of plants and fungi. This application note presents a flexible automated solution for the isolation of plant genomic DNA without compromising yield or purity; a result of Tecan and MACHEREY-NAGEL have joining forces.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PurificationPurification techniques are essential for isolating compounds of interest from complex mixtures, such as proteins, nucleic acids, and chemicals. These techniques are critical in biochemistry, drug development, and environmental studies. Explore purification systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.Plant SciencePlant science studies the biology of plants, including genetics, physiology, ecology, and biotechnology. Research in this field is essential for improving crop yields, disease resistance, and sustainability. Explore plant science tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.PCR PurificationBlottingSample ProcessingSample processing is required to prepare samples prior to analysis. Technologies used for sample processing include homogenizers, grinders, mills and mixers.Genome EditingGenome editing involves altering the DNA of an organism, which holds promise for disease treatment, agriculture, and gene therapy. Techniques like CRISPR-Cas9 are revolutionizing this field, enabling precise genetic modifications. Explore genome editing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Getting High Yields of gDNA from Plant Samples; MACHEREY-NAGEL and Tecan