ResourceLife Sciences
Get the Whole Story: Combine T<sub>m</sub> and T<sub>agg</sub> with Sizing and Polydispersity on UNcle
7 Jan 2018This application note describes how you can use UNcle to get even more information out of a simple Tm and Tagg screen, by opting to collect DLS data at the beginning and end of a thermal ramp. Without adding any more sample volume or set up time to your workflow, get high-resolution information about the size and polydispersity of your samples from the same instrument. Learn about any red flags with your formulations or unstable constructs, and use the sizing information as an additional clarification for samples that display odd behaviors in the thermal ramp measurements.
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Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.Dynamic Light ScatteringDynamic light scattering is used to measure the size distribution of particles in suspension by analyzing the way light scatters off them. It is commonly used in materials science, nanotechnology, and protein characterization. Browse our peer-reviewed product directory to find the best dynamic light scattering systems, compare products, check reviews, and get pricing directly from manufacturers.Protein StabilityProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.