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Gel-Free Size Selection Using SPRIselect For Next Generation Sequencing

Beckman Coulter Diagnostics
25 Jun 2013

Gel size selection for Next Generation Sequencing (NGS) fragment library preparation has been the standard process for achieving size selection. Aside from being tedious, gel size selection creates additional preparation time for scientists forcing them to purify the desired fragment sizes from the gel creating a process that can only be low throughput. In this poster read how SPRIselect, from Beckman Coulter, helps to alleviate this problem.

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Biomek® 4000 Laboratory Automation Workstation

Beckman Coulter Life Sciences

Biomek Liquid Handlers enable you can spend less time on manual process and more time on powerful discoveries. Beckman Coulter Life Sciences is a leader when it comes to providing solutions and support that cover the full spectrum of the NGS sample preparation workflow with reagents and automation. Throughput options allow you to choose the right solution for your laboratory.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Size SelectionDNA Fragment SizingCancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

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Gel-Free Size Selection Using SPRIselect For Next Generation Sequencing