ResourceLife Sciences
Fura-2 QBT Calcium Kit from Molecular Devices: A New Homogenous Fura-2 Calcium Assay
4 Nov 2014Fura-2 dye has long been considered an important tool to measure calcium mobilization in cellular imaging,
GPCR-mediated intracellular calcium flux, and ion channel activation. This application note demonstrated the use of the Fura-2 QBT™ Calcium Kit from Molecular Devices® which incorporates proven quench based technology with a ratiometric Fura-2 calcium indicator to provide a homogenous assay to minimize cell based variability, while increasing throughput through elimination of cell washing prior to detection. In addition, the Fura-2 QBT Calcium Kit excites in the ultraviolet spectrum, enabling researchers to mitigate fluorescent compounds that are excited at 488 nm.
Links
Tags
UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CalciumIon ChannelUVUltraviolet (UV) light is used in a variety of applications, including disinfection, spectrophotometry, and molecular biology. UV light can damage DNA, making it a powerful tool in sterilization and in studies of mutagenesis and gene repair. Explore UV-related products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.