ResourceSpectroscopy
Fully Automated Sitting Drop Protein Crystallization with CyBi-CrystalCreator
30 Oct 2012Various proteins have been successfully crystallized with CyBio’s CyBi®-CrystalCreator using the JBScreen Basic HTS Kit from Jena Bioscience. In this application note the high precision of the automated system and reliable choice of crystallization solutions were reflected in a very high rate of crystallization hits. The use of intuitive graphical software and ready-made buffer solutions were shown to facilitate a straightforward and extremely fast setup of the crystallization experiment described here.
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X-ray CrystallographyX-ray crystallography is an analytical technique used to determine the arrangement of atoms in a crystal. Monochromatic x-rays are produced from a synchrotron or x-ray generator. An x-ray crystallography system uses a detector to measure the x-ray diffraction from the crystal. The information is used to generate a 3D image of the crystal.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.CrystallizationStructural Biology