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Full Characterization of Heterogeneous Antibody Samples Under Denaturing and Native Conditions on the Q Exactive BioPharma Mass Spectrometer

Thermo Fisher Scientific
6 Nov 2017

This application note demonstrates the capabilities of the Thermo Scientific™ Q Exactive™ BioPharma mass spectrometry platform, particularly the High Mass Range (HMR) Mode for the characterization of antibody samples. The three different operational modes of the BioPharma option are described and application data are presented for the major application workflows.

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Thermo Scientific™ Q Exactive™ BioPharma Platform

Thermo Fisher Scientific

  The Thermo Scientific™ Q Exactive™ Plus and Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ mass spectrometers adds superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to the most powerful benchtop peptide mapping instruments available. When combined with Thermo Scientific™ BioPharma Finder™ software it provides a complete integrated hardware and software solution for BioPharma characterization.   The Thermo Scientific™ Q Exactive™ BioPharma platform offers distinct operational modes that have been optimized for the top 3 protein characterization workflows: High Mass Range (HMR) mode was designed specifically for intact therapeutic protein workflows with the aim of providing ease of use an flexibility as needed. HMR mode is optimized for both conventional intact monoclonal antibody analysis performed in denaturing conditions, as well as native MS analysis which is ideal for antibody drug conjugates (ADCs) and other heterogeneous antibody samples Protein mode on the Q Exactive HF is a powerful combination that has set the standard for the highest quality isotopically resolved data achievable on a benchtop mass spectrometer. Deconvolution of subunit MS spectra is easy and powerful with Thermo Scientific™ BioPharma Finder™ Software Peptide mapping with Standard mode is state-of-the-art with the Q Exactive BioPharma, which is a culmination of decades of leadership in the development of data dependent acquisition (DDA)  

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.TherapeuticsTherapeutics are treatments designed to alleviate or cure diseases. These include pharmaceuticals, biologics, and gene therapies, which work by targeting specific disease mechanisms. Advances in personalized medicine and biologics offer new hope for patients with conditions that were previously difficult to treat. Browse our peer-reviewed product directory to find therapeutic solutions for various diseases; compare products, check user reviews, and get pricing directly from manufacturers.

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Full Characterization of Heterogeneous Antibody Samples Under Denaturing and Native Conditions on the Q Exactive BioPharma Mass Spectrometer