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Formation of Spheroids and Suppression of Adhesion by Adherent Growing Cells in inertGrade™ Microplates

BRANDTECH® Scientific, Inc.
18 Dec 2014

Suppression of the cell adhesion that is mediated by integrin is of significant importance in many experimental procedures, such as the production of embryonic bodies, self-assembled 3D cultures, and dose-response relationships in cultivated tumor spheroids. This application note demonstrates that BRANDplates® inertGrade™ surfaces suppress the adhesion and spreading of adherent growing cells.

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BRANDplates Cell Culture Microplates - inertGrade™

BRANDTECH® Scientific, Inc.

BRANDplates® inertGrade™ 96-well plates are especially suited for cell culture applications when adhesion is not desired. These plates are specifically designed for the cultivation of suspension, tumor, and stem cells, as well as other spheroid or 3-dimensional tissue culture models. They feature a surface that reduces cell adhesion and protein absorption to minimize enzyme and cellular activation. All BRANDplates® inertGrade™ microplates are sterile according to Ph. Eur. and USP 29, SAL10-6, and are alphanumerically coded with orange embossing for easy plate and well identification. Available clear, white, and black, with U- F- or C-bottom, as well as white or black with transparent bottom, to suit your needs.  

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.

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Formation of Spheroids and Suppression of Adhesion by Adherent Growing Cells in inertGrade™ Microplates