ResourceSpectroscopy
Fluorescent Measurements using a Stopped-Flow Accessory with an ISS PC1 Fluorimeter
6 Oct 2011In this application note the SFA-20 stopped-flow accessory is demonstrated with an ISS PC1 fluorimeter to test their combination in the acquisition of fluorescent measurements. With this accessory, the collection of kinetic data was shown to be quite straightforward in terms of interfacing to the ISS instrument and was capable of producing good quality, reproducible traces. The method was shown to be economical in terms of sample consumption; a shot volume of 300 μl was used in the tests.
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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.