ResourceLife Sciences
Fluorescent macromolecules and nanoparticles: Characterization of molar mass, size and charge
29 Jan 2024In this whitepaper, Waters | Wyatt Technology highlight the prevalence of fluorescent macromolecules and nanoparticles in various fields of biological, chemical, and medical research and development. The paper focuses on how the SEC-MALS, DLS, and PALS detectors effectively address the challenges posed by fluorescence. It also introduces additional technologies capable of handling such samples and presents several case studies for illustration.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.NanotechnologyNanotechnology, or nanotech, is an engineering technique using molecular scale functional systems. Applications of nanotechnology include medicine and medical devices, electronics, air and water purification, food science and energy production.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Dynamic Light ScatteringDynamic light scattering is used to measure the size distribution of particles in suspension by analyzing the way light scatters off them. It is commonly used in materials science, nanotechnology, and protein characterization. Browse our peer-reviewed product directory to find the best dynamic light scattering systems, compare products, check reviews, and get pricing directly from manufacturers.NanoparticlesNanoparticles are between 1-100nm in size. Nanoparticles can be used for a wide variety of applications including biomedical, catalysis and electronics.