Fluorescent Immunodetection Using the SNAP i.d.™ Protein Detection System

5 Aug 2015

Recent advances in fluorescent dye chemistry and blotting membranes coupled with improvements in instrumentation have accelerated the application of fluorescence detection methodologies to western blotting. The SNAP i.d. Protein Detection System has been developed to substantially shorten the time required for fluorescent immunodetection through the use of vacuum. This application noted discusses how to the SNAP i.d. Protein Detection System can be optimized for this purpose.

SNAP i.d. 2.0 IHC System

Merck

Issues with Your Tissues? Process all Your Slides in a SNAP for Streamlined IHC. The SNAP i.d.® 2.0 Protein Detection System for Immunohistochemistry (IHC) introduces a new capability to the innovative, vacuum-driven SNAP i.d.® 2.0 system. The IHC slide holders allow you to block, probe, and stain up to 12 tissue slides per side (24 slides if both sides are used). Reduced handling time and multiple-slide processing make this system ideal for when you are optimizing antibody conditions and protocols.With two individually controlled sides, the system base allows for independent, vacuum-driven processing of either one or two IHC frames. Each of the IHC frames can process between 1 to 12 glass slides through independent vacuum ports.Each slide holder has an injection/recovery port that enables the manual addition, as well as the removal and recovery, of small volumes of antibodies or reagents; reagents can also be flushed using the vacuum feature if conservation is not a priority.Benefits: Eliminates the need for pap pens Antibodies can be collected and reused Slide handling time is significantly decreased Less time spent on wash steps Parallel processing of multiple slides Features: Flexibility of multiple slide configurations enables the processing of 1 to 24 slides at a time Compatible with standard IHC slides and protocols Compatible with diverse tissue preparations including formalin-fixed or fresh frozen samples Intuitive format Incorporates blocking, washing, and antibody incubation and labelling steps Systematizes handling multiple slides without the cost of automation Test tracker feature on frame cover helps keep track of IHC steps

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SNAP i.d. 2.0 System

Merck

The SNAP i.d. Protein Detection System revolutionizes the quality of your blots every time — in record time! Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d. system applies a vacuum to actively drive reagents through the membrane. This novel method allows you to optimize your blotting conditions in record time for maximum results. The SNAP i.d. system minimizes overblocking by using lower concentrations of blocking agents. In addition, more effective wash steps remove unwanted contaminants from the membrane. With the SNAP i.d. system, you’ll achieve incredibly low background, high signal-to-noise ratios, reproducibility from blot to blot, and sensitivities that are the same or better than traditional immunodetection techniques. Compatible with your favorite membrane and detection methods, the SNAP i.d. system produces higher quality blots than traditional western blot methods -- in less than 30 minutes. SNAP i.d. System Features: Dynamic – vacuum actively drives reagents through blotting membrane. Quality – equal or better signal-to-noise ratios than standard western blotting. Fast – reduces immunodetection time from 4 hours to 30 minutes. Simple – incorporates blocking, washing, and antibody incubation steps. Compatible – works with standard gel sizes and protocols. Efficient – optimizes your protocol with a new antibody in 30 minutes.

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.ImmunofluorescenceFluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.